Kariko and Weissman Study 2015: They discovered LNP/mRNA injection used during the development of the COVID-19 vaccines did not stay in the muscle, describing lipid distribution altered by injection.
Kariko and Weissman Study 2015: They discovered LNP/mRNA injection used during the development of the COVID-19 vaccines did not stay in the muscle, describing lipid distribution altered by injection.
Why weren't the COVID-19 mRNA vaccine consumers and providers warned that biodistribution was able to be significantly changed/manipulated via parameters of the injection/administration?
The "LNP files" page links to a private page which I was not able to access.
The "Expression Kinetics" rat study article is: Pardi, Tuyishime, Muramatsu, Kariko, Mui, Tam, Madden, Hope Drew Weissman, J Control Release, 2015-08-08 "Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624045/ .
"When mRNA-LNPs were injected intramuscularly and intratracheally, similar to intravenous and intraperitoneal deliveries, a large portion of the luciferase activity was detectable in the liver, demonstrating systemic spread of the nanoparticles."
It may be difficult to reliably extrapolate from mice to humans, but in the absence of specific guidance for mRNA quasi-vaccine injection technique, and especially the lack of requirement of aspirating the needle to reduce the chance that the fluid is injected into a blood vessel, it is reasonable to expect that this uncontrolled injection regime would result in significant delivery of the lipid nanoparticle contents to distant parts of the body, including the liver and potentially all other organs, especially the heart.
Also of interest is this 2023-10-11 article https://www.mdpi.com/2076-393X/11/10/1580 in which mice given intramuscular injections of LNPs containing mRNA OR DNA plasmids encoding a gene which, when expressed to create protein, causes the cell to glow. The LNPs were made with different formulations.
"Following intramuscular injection in mice, we determined that RNA-LNPs formulated with either SM-102 or ALC-0315 lipids were the most potent (all p-values < 0.01) and immunogenic (all p-values < 0.05), while DNA-LNPs formulated with SM-102 or ALC-0315 demonstrated the longest duration of signal. Additionally, all LNP formulations were found to induce expression in the liver that was proportional to the signal at the injection site . . ."
Since we can't rule out the possibility that human quasi-vaccine injections, at least in some instances, were functionally intramuscular, we can reasonably assume that their intended mRNA (modified messenger RNA) payloads, and their contaminants (fragments and/or erroneous mRNA and fragments of DNA) will be delivered to and alter the function of cells in many and perhaps all parts of the body often enough to cause effects which were not intended by the vaccine designers.
Amazing work- thank you!
The "LNP files" page links to a private page which I was not able to access.
The "Expression Kinetics" rat study article is: Pardi, Tuyishime, Muramatsu, Kariko, Mui, Tam, Madden, Hope Drew Weissman, J Control Release, 2015-08-08 "Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624045/ .
"When mRNA-LNPs were injected intramuscularly and intratracheally, similar to intravenous and intraperitoneal deliveries, a large portion of the luciferase activity was detectable in the liver, demonstrating systemic spread of the nanoparticles."
It may be difficult to reliably extrapolate from mice to humans, but in the absence of specific guidance for mRNA quasi-vaccine injection technique, and especially the lack of requirement of aspirating the needle to reduce the chance that the fluid is injected into a blood vessel, it is reasonable to expect that this uncontrolled injection regime would result in significant delivery of the lipid nanoparticle contents to distant parts of the body, including the liver and potentially all other organs, especially the heart.
Also of interest is this 2023-10-11 article https://www.mdpi.com/2076-393X/11/10/1580 in which mice given intramuscular injections of LNPs containing mRNA OR DNA plasmids encoding a gene which, when expressed to create protein, causes the cell to glow. The LNPs were made with different formulations.
"Following intramuscular injection in mice, we determined that RNA-LNPs formulated with either SM-102 or ALC-0315 lipids were the most potent (all p-values < 0.01) and immunogenic (all p-values < 0.05), while DNA-LNPs formulated with SM-102 or ALC-0315 demonstrated the longest duration of signal. Additionally, all LNP formulations were found to induce expression in the liver that was proportional to the signal at the injection site . . ."
Since we can't rule out the possibility that human quasi-vaccine injections, at least in some instances, were functionally intramuscular, we can reasonably assume that their intended mRNA (modified messenger RNA) payloads, and their contaminants (fragments and/or erroneous mRNA and fragments of DNA) will be delivered to and alter the function of cells in many and perhaps all parts of the body often enough to cause effects which were not intended by the vaccine designers.
I found this page via: https://phillipaltman.substack.com/p/shocking-revelations-on-the-covid
Notice how many of those who speak against the vaccines are focusing on distractions from this fact about the LNPs.
They get sucked up into the irony that a toxic platform of LNPs is toxic whether it has foreign DNA, spike protein issues, etc or not.
I think that these distractions are being revealed in order to hide that the platform, the lipids, are the real cause of a myriad of issues.
Besides, Karikó developed a method do get rid of the dsRNA and DNA remainders:
https://twitter.com/Sabisteb/status/1709911188329668980
Moderna and BioNTech/Pfizer left that step out.
I worte a substack on the resulting problems do to insertional mutagenesis
https://drbine-substack-com.translate.goog/p/integriert-die-plorre-nun-oder-nicht?_x_tr_sl=auto&_x_tr_tl=en&_x_tr_hl=de&_x_tr_pto=wapp
Furthermore it's even boldly stated in a BioNTech patent that biodistribution is a problem
https://twitter.com/Sabisteb/status/1710307665472016476
The Nobel Laureate Weissman knew it also
https://twitter.com/Sabisteb/status/1709939931722956991
Karikó was a senior vice president at BioNTech: https://www.linkedin.com/in/katalin-karik%C3%B3-a6b457a/?originalSubdomain=de
Why didn't she insist on a HPLC/FPLC purification step?
To the European regulators this was NEVER a secret as it is openly stated in the EPAR documents:
"Overall, only a
relatively small fraction of the administered
mRNA-1647 dose distributed to distant tissues
(ie, lung, liver, heart, kidney, axillary distal
lymph nodes [bilateral pooled], proximal
popliteal and inguinal lymph nodes [bilateral
pooled], spleen, brain, stomach, testes, eye,
bone marrow femur [bilateral pooled],
jejunum [middle region], and injection site
muscle), and the mRNA constructs did not
persist past 1 to 3 days in tissues other than
the injection site, lymph nodes, and spleen
where it persisted in general 5 days."
https://www.ema.europa.eu/en/documents/rmp-summary/spikevax-previously-covid-19-vaccine-moderna-epar-risk-management-plan_en.pdf
At least the EMA knew officially it would go into every organ.
Pfizer tried to hide it, Moderna though was honest.
The problem ist the measuremt of the half life which seems to be tricky and not very accurate:
https://pubmed.ncbi.nlm.nih.gov/31960607/